Practical GK-13

Protein design

Aims

• To reinforce the concepts of protein stability.

Objectives

After this practical you will:

• have examined and considered the stability of a computationally designed protein.

Exercises

1. Protein Data Bank entry 1QYS contains an crystal structure of the computationally designed protein Top7. Suggest 2 amino acid residue mutations that, for different reasons, might reduce the stability of Top7, or even prevent it from adopting a stable fold. Suggest another mutation that would be unlikely to affect the protein's stable fold. Give reasons to justify your choices.