Bioinformatics (2014/2015)

Lecture GK-5

Sequence alignment

Aims

Objectives

After this lecture you will be able to:

Supplementary Material

The lecture handout, featuring some of the lecture slides, is available on-line (one per page, four per page).

Tutorial on The Statistics of Sequence Similarity Scores by Stephen Altschul, author of BLAST.

The method for deriving BLOSUM matrices is described in: Henikoff, S. and Henikoff, J.G. (1992) Amino acid substitution matrices from protein blocks. Proc Natl Acad Sci USA, 89, 10915-10919 (PubMed).

There is an overview of BLAST and substitution matrices in: Pertsemlidis, A. and Fondon, J.W. (2001) Having a BLAST with bioinformatics (and avoiding BLASTphemy), Genome Biol., 2, reviews2002.1-reviews2002.10.


Last Modified: 11 February 2015 by Graham Kemp